Fig. 1: Dynamic transcriptomic changes in multipotent mesenchymal progenitors from human adipose tissue undergoing adipogenic differentiation.
From: Wnt signaling preserves progenitor cell multipotency during adipose tissue development
a, Mesenchymal progenitor cells expanded from adipose tissue explants in three-dimensional culture (top), plated and grown to confluence in two-dimensional culture dishes (bottom; scale bar, 50 μm). b, Images of progenitors induced toward adipogenic, chondrogenic or osteogenic cell fates for 10 d. Adipogenic-induced cells were stained with Oil Red O, chondrogenic-induced cells with Alcian blue 8GX and osteogenic-induced cells with Alizarin red S. Scale bar, 50 μm. c, Marker genes for progenitors differentiated toward adipogenic, chondrogenic and osteogenic lineages identified using their transcriptomic profile. Bars are means of technical replicates from n = 1 wells subjected to the indicated differentiation cocktails. TPM, transcripts per million. d, Schematic of the adipogenesis time-course study. e, Representative images of mesenchymal cells induced toward adipogenic fate for 0, 3, 7 and 14 d. Scale bar, 50 μm. f, Scatter-plot of the first two principal components of bulk RNA-seq results from two independent cultures, each from two independent donors, separately expanded and used to obtain RNA at 0, 3, 7 or 14 d after adipogenic induction. PCA was performed on the expression of the top 1,000 most variable genes across all n = 16 samples. g, Volcano plots of the differential gene expression analysis results between consecutive time points. h,i, Time courses of ADIPOQ and LEP and of mesenchymal progenitor markers THY1 (CD90), ENG (CD105) and NT5E (CD73).
