Extended Data Fig. 4: Dominant negative TCF expressing progenitors have adipogenic fate preference.
From: Wnt signaling preserves progenitor cell multipotency during adipose tissue development
a, c, e, g, Representative fluorescence microscopy images of 7-day adipogenic-induced cells from 4 separate donors, transduced with lentiviral vectors expressing control or dominant-negative TCF and mCherry reporter. The transduction was performed 72 hours prior to adipogenic induction. Cells were stained with LipidTOX green neutral lipid stain prior to imaging. Scale bars, 50 μm. b, d, f, h, Quantification of adipocytes and non-adipocytes (inferred by positive and negative LipidTOX signal) among the transduced cells (inferred by positive mCherry signal).
