Fig. 2: Single-cell RNA-seq of induced adipose progenitors.
From: Wnt signaling preserves progenitor cell multipotency during adipose tissue development
a, Schematic of the early adipogenesis single-cell transcriptomic profiling study. b, PCA projection of the single-cell profile of 6,615 cells (3,226 non-induced; 3,430 adipogenic-induced; mean number of genes per cell, 3,382). c, Clustering of the single cells with Louvain algorithm. d, Inference of developmental trajectory with RNA velocity. Red and blue colored cells represent clusters at the terminals of two projected fate trajectories. e, Expression of representative cluster 5 (ADIPOQ, PLIN1, LPL and FABP4), cluster 3 (MGP, CTHRC1 and DCN) and mesenchymal progenitor cell marker genes. f, Volcano plot comparing cluster 3 and cluster 5 cell gene expression, with differentially expressed genes highlighted. Tested features were limited to genes detected in >25% cells in at least one of the clusters. Differentially expressed genes were defined as those with log2 fold change > 1 and adjusted P value < 0.001. g, Top six significantly enriched gene sets of genes upregulated in cluster 5/ADIPOQ+ cells. h, Top six significantly enriched gene sets of genes upregulated in the cluster 3/SWAT cells. i, Dot-plot of the canonical Wnt target genes that were significantly upregulated in cluster 3 cells. j, Dot-plot of the detected Wnt ligands and core Wnt pathway members.
