Fig. 5: Early canonical Wnt signaling induced by adipogenic stimuli coincides with progenitor cell fate.
From: Wnt signaling preserves progenitor cell multipotency during adipose tissue development
a, Time-course measurement of canonical Wnt activity upon adipogenic induction on two separate donor cells expressing STF luciferase reporter. b, Representative RNAscope images of 1-, 2- and 3-d adipogenic-induced cells probed for FABP4 (red), MGP (green) and 4,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar, 50 μm. c,d, Quantitation of MGP- and FABP4-positive cell number (c) and total signal intensity (d) from RNAscope images of 1-, 2- and 3-d adipogenic-induced cells. Each data point represents quantification from one distinct field on the coverslip (n = 10). Data are shown as mean ± s.d. One-way ANOVA was used for statistical analysis. e, Scatter-plots of FABP4 and MGP intensity of individual cells. Each dot represents one cell. f, Model depicting potential sequence of events upon adipogenic induction, consistent with data presented. Canonical Wnt activity is upregulated early upon receiving adipogenic signal, followed by fate determination leading to emergence of SWAT cells and early adipocytes. SWAT cells can regain proliferative capacity and multipotency.
