Extended Data Fig. 4: Expression of ETC components in EAE neurons.
(a) Top: Schematic of the experiment, analysis of same data sets as shown in Fig. 3. Bottom: Relative abundance of individual ETC complex components in neuronal mitochondria. Average shown as color-coded log2(EAE/Control) for acute and chronic EAE compared to respective controls. (b) In situ analysis of axonal COX IV activity using in situ histochemical assay in Thy1-OFP EAE spinal cords. Top: Schematic of the experiment. Bottom: Confocal image of control and EAE stage 1 axons, OFP (red) and COX IV (arrow heads indicate mitochondria as dark areas, as fluorescence of OFP is quenched by reaction product of COX IV assay). (c) Quantification of COX IV activity signal’s occupancy of OFP axon area on axonal level. Mean ± s.e.m.; n = 180 axons from nine mice for control and 208 axons from nine mice for EAE using a two-tailed, Mann-Whitney test, p = 5.2 × 10-12. Scale bar: 25 μm in b. ****, p < 0.001. See source data for individual data points and further statistical parameters. Illustration created with BioRender.
