Extended Data Fig. 5: Viral overexpression of Idh3a in EAE neurons. | Nature Metabolism

Extended Data Fig. 5: Viral overexpression of Idh3a in EAE neurons.

From: Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions

Extended Data Fig. 5

(a) Schematic of experiment, analysis of same data sets as shown in Fig. 6. (b) Confocal images of spinal cord sections of a Thy1-PercevalHR mouse (green) that was injected with rAAV.hSyn:Idh3a-tdTomato (tdTomato, tdTom, red). Bottom row shows immunostainings for Idh3a, with details highlighting, left, a tdTomato-negative (tdTom−) and, right, a tdTomato-positive (tdTom+) neuron with Idh3a overexpression. (c) Expression level of Idh3a in tdTomato-negative (tdTom−) and tdTomato-positive (tdTom+) neurons (mean ± s.e.m.; n = 266 tdTom− neurons and 803 tdTom+ neurons using two-tailed, Mann-Whitney test, p < 0.001). Scale bar: 25 μm (top) and 10 μm (bottom) in b. ****, p < 0.001. See source data for individual data points and further statistical parameters. Illustration created with BioRender.

Source data

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