Fig. 3: ETC depletion and TCA cycle imbalance in neurons during EAE.
a, Experimental design for combined AAV/MitoTag-based proteomic analysis of neuronal mitochondria in EAE. b, Confocal image of GFP expression (green) in rAAV9.hSyn:Cre transduced neurons (NeuN; red) in a control and EAE MitoTag mouse spinal cord. c, Annotations of the most downregulated pathways (Reactome59,60, v.7.4) in MitoTag proteomes of neuronal mitochondria in acute EAE. Dot size indicates min–max scaled mean protein expression level ranged from 0 to 1. NES, normalized enrichment score. d, Relative abundance of the TCA cycle and ETC components in neuronal mitochondria. Average shown as color-coded log2(EAE/control) for acute and chronic EAE compared to respective controls. e, Rank of TCA cycle (orange) and ETC (green) proteins according to log2FC expression in acute (top) and chronic (bottom) EAE. f, Correlation between neuronal transcript and protein levels of TCA cycle and ETC proteins in EAE. Translatomic data were re-analyzed from Schattling et al.27. The acute cohort was collected from six control and five EAE acute mice and the chronic cohort was collected from five control and five EAE chronic mice. Scale bars, 25 μm in b. See source data for individual data points and further statistical parameters. See source data for individual data points and further statistical parameters. Illustration created with BioRender.
