Extended Data Fig. 1: SYNB strain design and key engineering features.
From: Efficacy and safety of a synthetic biotic for treatment of phenylketonuria: a phase 2 clinical trial
SYNB1618 (A) and SYNB1934 (B) are shown. Abbreviations; LAAD = L-amino acid deaminase from Proteus mirabilis; laad = gene for LAAD. PAL = phenylalanine ammonia lyase from Photorhabdus luminescens; pal = gene for PAL; mPAL = modified phenylalanine ammonia lyase with higher potency. mpal = gene encoding mPAL. PheP = high-affinity phenylalanine transporter; pheP = gene encoding high-affinity phenylalanine transporter; PFNR = promoter regulated by fumarate and nitrate reductase regulator; Ptac = synthetic promoter controlling PAL expression and regulated by the LacI transcriptional repressor; PBAD = promoter for laad, regulated by arabinose; ΔdapA = deletion of dapA gene leading to diaminopimelate auxotrophy; Phe = phenylalanine, PP = phenylpyruvate; TCA = trans-cinnamic acid.
