Extended Data Fig. 7: Protein polarity of shed cells.
From: The cellular states and fates of shed intestinal cells
a-b. Intact mouse Jejunum tissue stained with an antibody against CD73 (green) and Dapi (grey), show apical polarity of CD73 at the villi tip. Other CD73 positive cells are monocytes/macrophages. Scale bar in a and b are 25μm and 10μm respectively. c. Dissociated mouse Jejunum tissue stained with anti EpCAM (magenta), anti CD73 (green) and Hoechst (grey). Both EpCAM and CD73 show cell polarity. d. Faecal wash cells stained with anti EpCAM (magenta), anti CD73 (green) and Hoechst (grey). Both EpCAM and CD73 show loss of cell polarity. Images are representative of at least 3 independent experiments.
