Extended Data Fig. 1: Differential gene expression between faecal washed to tissues.
From: The cellular states and fates of shed intestinal cells
a. Examples of genes that are only expressed in the small intestine but not in the colon tissue, yet are still observed in colonic faecal washes. These genes demonstrate a slow decline in gene expression of shed intestinal cells. Y axis is log10(Normalized RNA Expression), n = 10 mice. Medians are marked with white dot. Means are marked with x and connected with a dashed line. b, c Volcano plot showing differentially expressed genes between faecal washes and tissues in small (b) or large (c) intestine, each dot is a gene, x-axis is the log2- ratio of expression between faecal wash and tissue, y axis is –log10 (q value), where p value is computed using 2-sided Wilcoxon rank-sum tests and q- values computed using Benjamini- Hochberg FDR correction. Genes with corresponding q-values below 0.05 and fold change greater than 1.5 are marked in red or blue for faecal wash or tissue elevated genes respectively. Names of representative up-regulated genes are shown in red or blue for faecal wash or tissue respectively.
