Extended Data Fig. 2: Altered microbial communities are mainly responsible for gut ammonia production and depressive-like behaviors.
From: Gut microbiome-derived ammonia modulates stress vulnerability in the host
a, Timeline of oral broad-range antibiotics for 7 days and tissue collection. b, The levels of ammonia in the blood, feces and mPFC of vehicle and Abx-treated mice (n = 10 mice; Blood: P < 0.0001, Feces: P = 0.0023; mPFC: P = 0.0444). c, The levels of urease in the feces of vehicle and Abx-treated mice (n = 8 mice, P = 0.0008). d, Schematic of the FMT study: Abx-treated mice were colonized with fecal samples from control or CSDS donors followed by behavior tests. e, Behavioral tests of the recipient mice in the SIT, SPT, FST and TST. For SIT, n = 20, 18 mice, P = 0.0204; For SPT, n = 20, 18 mice, P = 0.0136; For FST n = 20, 17 mice, P = 0.0037; For TST, n = 10 mice, P = 0.0007. f, Behavioral tests of the recipient mice in the OFT (n = 10, 8 mice). Data are the mean ± s.e.m., *P < 0.05, **P < 0.01, ***P < 0.001. Statistical differences were determined by two-tailed unpaired Student’s t-test. The statistical details are provided in Supplementary Table 1.
