Extended Data Fig. 3: CSDS alters gut microbiota and urease-positive bacteria.
From: Gut microbiome-derived ammonia modulates stress vulnerability in the host
a, α-diversity index Shannon of gut microbiome of control and CSDS mice by two-sided Wilcoxon rank-sum test (n = 6 mice; whiskers indicate minimum to maximum values; the minima, first quartiles, median, third quartiles and maxima of control group are 7.671, 7.676, 7.763, 7.855, 7.938, CSDS group are 7.244, 7.278, 7.318, 7.430, 7.465; P = 0.026). b, Principal co-ordinates analysis (PCoA) plot of β-diversity (unweighted unifrac no label) in the feces (n = 6 mice). c, Taxonomic abundance of bacteria at phylum level of control and CSDS mice. Data were showed as relative abundance (%) of top 15 most abundant phylum in each group by Wilcoxon rank-sum test (n = 6 mice). d, Histogram of the LDA scores for differentially abundant KEGG pathway at level 1 and level 2 analyzed by LEfSe analysis (n = 3 mice, P < 0.05, LDA > 3). e, Urease-related pathways enriched from metagenomic pathway at level 3 (n = 3 mice). f, Relative abundance of urease in arginine biosynthesis pathway of control and CSDS-exposed mice (n = 3 mice, P = 0.0352). g, The levels of arginase in the feces and colon of control and CSDS-exposed mice (n = 8 mice). h, The bacterial taxa from taxonomic classification of urease genes as determined by sequence abundance. Data are presented as median with interquartile range (a) and mean ± s.e.m. (f, g), *P < 0.05. Statistical differences were determined by two-tailed unpaired Student’s t-test (f, g). The statistical details are provided in Supplementary Table 1.
