Fig. 4: Glycolysis in cortex glia is necessary for MTM and LTM.
From: Glycolysis-derived alanine from glia fuels neuronal mitochondria for memory in Drosophila
a, PFK KD in adult cortex glia impaired memory after single-cycle training (n = 12, F2,33 = 8.26, P = 0.001) and spaced training (n = 12, F2,33 = 14.21, P = 0.000035), but did not affect memory after single-cycle training followed by cold shock (n = 12, F2,33 = 1.99, P = 0.15) or massed training (n = 12, F2,33 = 3.16, P = 0.055). b, Single-cycle training elicited a faster pyruvate accumulation in MB neuron axons following sodium azide application (5 mM) compared to non-associative unpaired training (n = 12, t22 = 2.75, P = 0.012). PFK KD in adult cortex glia impaired the single-cycle induced increase in pyruvate accumulation in MB neuron axons following sodium azide application (n = 13, t24 = 0.94, P = 0.36). c, A mild decrease in both ALAT and PFK expression in cortex glia impaired memory after single-cycle training, whereas the mild induction of RNAi against ALAT or PFK alone did not affect memory (n = 18, F4,85 = 5.08, P = 0.001). All data are presented as mean ± s.e.m. Asterisks (*P < 0.05; **P < 0.01; NS, not significant, P > 0.05.) illustrate the significance level of a two-sided t-test or of the least significant pairwise comparison following one-way ANOVA.
