Extended Data Fig. 2: Behavioral controls of PFK and LDH KD in MB neurons (related to Fig. 2).
From: Glycolysis-derived alanine from glia fuels neuronal mitochondria for memory in Drosophila
a. PFK knockdown (KD) in adult MB neurons using a second RNAi did not affect memory after single-cycle training (n = 10, F2,27 = 0.85, P = 0.44) or single-cycle training followed by cold shock (n = 10, F2,27 = 0.35, P = 0.71). b. LDH knockdown in adult MB neurons using a second RNAi did not affect memory after single-cycle training (n = 15-16, F2,45 = 1.52, P = 0.23), single-cycle training followed by cold shock (n = 16, F2,44 = 0.76, P = 0.47), or spaced training (n = 12, F2,33 = 1.73, P = 0.19). c. Double KD of PFK and LDH in MB neurons did not affect memory after single-cycle training (n = 10, F2,27 = 1.37, P = 0.27) or after single-cycle training followed by cold shock (n = 10, F2,27 = 3.19, P = 0.06) All data are presented as mean ± SEM. Asterisks (*P < 0.05; **P < 0.01; ***P < 0.001; ns: not significant, P > 0.05) illustrate the significance level of two-sided t-test, or of the least significant pairwise comparison following 1-way ANOVA.
