Extended Data Fig. 2: Glut 2 expression and function in T cells.
From: The glucose transporter 2 regulates CD8+ T cell function via environment sensing
(a) Live and single cells from thymi of Glut2+and Glut2-chimera mice were analysed by flow cytometry. Representative dot-plots (left) and mean percentage of double-positive, CD4-single-positive, CD8-single-positive, and double-negative subsets (right) are shown (±SD; n = 3, N = 1). (b) T cells from the spleen and lymph nodes of Glut2+and Glut2-mice were analysed for expression of CD3, CD4, CD8, and FoxP3. Representative dot-plots (left) and mean percentage of CD4, CD8, and regulatory (Treg) subsets (right) are shown (±SD; n = 3, N = 1). (c-d) CD8+ and CD4+ effector memory T cells (Tem) and central memory T cells (Tcm) in ex-vivo T cells (c) and antibody-stimulated (6 days) naïve T cells (d) from Glut2+ and Glut2-. Representative dot-plots are shown on the top panel. The percentage of Tem and Tcm cells are shown on the bottom panel (±SD; n = 3, N = 1). Unpaired, two-tailed Student’s t-test. (e-f) Expression of the indicated molecules by naive (e) and antibody-activated (6 days) (f) CD4+ and CD8 + T cells was analysed by flow cytometry (n = 3).
