Extended Data Fig. 5: Regulation of Glut2 expression by Stomatin.
From: The glucose transporter 2 regulates CD8+ T cell function via environment sensing
(a) Expression of Stomatin mRNA by naïve and 3-day antibody-activated CD8 + T cells from Glut2+ or Glut2- mice was measured by RT-PCR. Stomatin gene expression was normalized to housekeeping gene tubulin (±SD; n = 3, N = 3). (b) T cells were antibody-activated in the presence of isotype control or anti-stomatin antibody at different concentrations before analysing Glut2 expression by flow cytometry. Mean data from a representative experiment are shown (±SD; n = 5, N = 2). (c) Purified CD8 + T cells were activated with plate-bound anti-CD3 (1 µg/ml) and anti-CD28 (5 µg/ml) monoclonal antibodies for 3 or 5 days before analysing surface expression of Glut2, stomatin and Gal-9 by decovolution microscopy imaging. (d) In some experiments, recombinant hGal-9 (30 nM) or vehicle control was added for 24 hours before staining. Bar charts show the corrected total cell fluorescence (CTCF) taken at the indicated time points. (±SD; c: n = 6, N = 2; d: n = 3, N = 2). a, Kruskal-Wallis test; b-c, One-way ANOVA; d, Unpaired, two-tailed Student’s T-test.
