Fig. 2: Glut2 contributes to CD8⁺ T cell function.

a, Mouse naive Glut2⁺ and Glut2⁻ CD8⁺ and CD4⁺ T cells were labelled with CFSE (5 μM) and stimulated with plate-bound anti-CD3 (1 µg ml⁻¹) and anti-CD28 (5 µg ml⁻¹) monoclonal antibodies for 3 d. Proliferation was assessed by flow cytometry. Histograms (grey indicates unstimulated cells) and mean data from a representative experiment (n = 3, N = 2) ± s.d. are shown. b,c, Glut2⁺ and Glut2⁻ chimera mice were primed and boosted (7 d apart) with IP injection of 750 µg ovalbumin protein plus 50 µg poly(I:C) adjuvant or adjuvant alone. After 7 d, T cells were separately harvested from mesenteric lymph nodes (dLN), inguinal and axillary (ndLNs) and the spleen. Expression of IFN-γ was assessed by flow cytometry. Representative dot plots are shown. Control injection with adjuvant alone and staining with an isotype-matched control antibody are shown on the right-hand side of each set. The mean number of IFN-γ⁺ cells from a representative experiment (n = 3, N = 2) is shown (±s.d.). Percentages are shown. d, Production of IFN-γ by mouse Glut2⁺ and Glut2⁻ CD8⁺ T cells that were antibody activated in Tc-1 or Tc-0 polarizing conditions (Methods) was measured by flow cytometry. n = 3–4, N = 2. e, Production of granzyme B by primed (b) mouse Glut2⁺ and Glut2⁻ CD8⁺ T cells from a representative experiment (±s.d.; n = 3, N = 2) is shown. Percentages are shown. f, T cells from Glut2⁺ and Glut2-deficient mice were activated by plate-bound CD3/CD28 antibodies, and differentiated toward Th0 and Th1 cells. CD107α was assessed by flow cytometry. Representative dot plots and histograms are shown (±s.d.; n = 10, N = 2). a–f, unpaired, two-tailed Student’s t-test.

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