Fig. 6: Stomatin and galectin-9 mediate regulation of Glut2 expression.
From: The glucose transporter 2 regulates CD8+ T cell function via environment sensing
a,b, Surface expression of stomatin (a) and Glut2 (b) by mouse CD44hi CD8+ T cells from blood, spleen and LNs was analysed for by flow cytometry. Histograms and mean data (±s.d.) from a representative experiment (n = 3, N = 2). c,d, Mouse naive CD8+ T cells were activated by antibodies in the presence of anti-stomatin or isotype control antibody before analysing Glut2 (c) and Glut1 (d) expression by flow cytometry. Histograms and mean data (±s.d.) from a representative experiment (n = 4, N = 2). e–h, Mouse naive CD8+ T cells were activated by antibodies for 3 d or 5 d before analysing surface expression of Glut2, stomatin, Gal-9 and CD3 by deconvolution microscopy. Representative deconvolution and channel co-localization images of stomatin/CD3 (e), stomatin/Glut2 (f), Gal-9/CD3 (g) and Gal-9/Glut2 (h) of non-permeabilized cells at day 0 (naive) and at day 3 and day 5 after activation are shown. Co-localization of different channels is indicated by the white areas in the cell images and presented as the percentage of cell volume. Bar charts show the mean percentage of cell volume (±s.d.) in at least n = 10 cells from a representative of N = 3 experiments. i,j, Mouse naive T cells were activated by antibodies in the presence or absence of recombinant Gal-9 (30 nM) before analysing surface expression of Glut2, stomatin and CD8+ by deconvolution microscopy. Representative deconvolution and channel co-localization images of stomatin/Glut2 (i) and Glut2/CD8 (j) are shown. Co-localization of different channels is indicated by the white areas in the cell images and presented as the percentage of cell volume. Bar charts show the mean percentage of cell volume (±s.d.) in at least n = 10 cells from a representative of N = 3 experiments. a,b,e–h, one-way ANOVA; c,d,i,j, unpaired two-tailed Student’s t-test.
