Extended Data Fig. 2: 31P ssNMR analysis of MLCL-induced structural and dynamic changes in the lipid membrane.
From: Anomalous peroxidase activity of cytochrome c is the primary pathogenic target in Barth syndrome
a, 1D 31P ssNMR static spectra of DOPC:MLCL (1:1) (blue) and DOPC:CL (1:1) (red) vesicles with bound cyt c. b, Analogous 31P NMR spectra in absence of cyt c. Panels (a,b) show that the presence of MLCL results in a narrower extra component, that is neither isotropic nor reflects a typical non-bilayer phase. As previously discussed23, these signals reflect MLCL in the liquid crystalline bilayer, undergoing distinct, increased dynamics (of the phosphate groups) compared to normal CL. c, 1D 31P ssNMR MAS spectra of DOPC:MLCL (1:1) at 10 kHz (blue curve) and 1.8 kHz (light blue curve) MAS rates. d, Zoomed-in region from (C) showing the isotropic peak (left) and first sideband (at 1.8 kHz MAS). Peak assignments are indicated, based on prior publications13,23. Note that in the side bands, the PC signals are strong, one MLCL signal is partly retained and one MLCL signal is attenuated or missing. This is consistent with the shown assignments and cited literature.
