Fig. 1: Addition of an MTS to ARCUS (mitoARCUS) localizes the nuclease to mitochondria. | Nature Metabolism

Fig. 1: Addition of an MTS to ARCUS (mitoARCUS) localizes the nuclease to mitochondria.

From: Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease

Fig. 1

a–c, Various m.3243A>G cybrid cell lines (cell lines A–D) were used for subsequent experiments. Cell line C (95% mutant) was used to evaluate ARCUS subcellular localization 24 h following mRNA nucleofection of ARCUS, either without a targeting sequence or with an MTS fused at the N terminus. m.3243A>G heteroplasmy analysis of various cybrid cell lines over 17 days in culture (a). Fluorescence microscopy of ARCUS lacking a targeting sequence (b). The experiment was performed once. Fluorescence microscopy of ARCUS with an MTS at the N terminus (mitoARCUS) (c). The experiment was performed once. ARCUS is shown in green, mitochondria (stained with MitoTracker) in red and nuclei (stained with Hoechst 33342) in blue; colocalization of ARCUS and MitoTracker is depicted in yellow (b,c).

Back to article page