Extended Data Fig. 2: Protein uptake and hydrolysis in ESCs.
From: Amino acid intake strategies define pluripotent cell states
(a) DQ-BSA signal in S/L + 2i ESCs (ESC-2) subjected to leucine (Leu) replete or depleted conditions. (b,c) Dextran uptake (b) and DQ-BSA hydrolysis (c) in S/L- and S/L + 2i-cultured ESCs (ESC-2). (d) Flow cytometry quantification of DQ-BSA signal in S/L-, S/L + 2i- and 2i/L (serum-free)-cultured ESCs. (e) Flow cytometry quantification of DQ-BSA signal in S/L- and S/L + 2i-cultured ESCs and PANC-1 pancreatic ductal adenocarcinoma cells. (f) Flow cytometry quantification of DQ-Ovalbumin signal in S/L- and S/L + 2i-cultured ESCs with or without pre-treatment with EIPA. (g,h) Population doublings of S/L- and S/L + 2i-cultured ESCs subjected to leucine (Leu) deficient conditions for 48 h in the presence or absence of EIPA (g) or chloroquine (h). Data are single measurements (b,c) or mean ± SD, n = 3 independent samples (d-h). Significance was assessed in comparison to S/L (d) or S/L + 2i (e) cells by one-way ANOVA with Sidak’s multiple comparisons post-test and two-way ANOVA with Sidak’s multiple comparisons post-test (g,h).
