Extended Data Fig. 5: Abrogation of weight loss effects of PAK4 deficiency under thermoneutral conditions.
From: p21-activated kinase 4 counteracts PKA-dependent lipolysis by phosphorylating FABP4 and HSL
Eight-week-old male Pak4 KO mice and their littermates (WT) were fed either an NCD or a 60% HFD for 16 weeks at 30 °C. a. Representative photographs of Pak4 KO and WT mice. b. Body weight changes in WT and Pak4 KO mice over 16 weeks of NCD or HFD feeding (n = 5). c. Gross examination of adipose tissues and weights of EAT, IAT, and BAT presented as percentages of body weight (BW) (n = 7). d. EAT sections were H&E stained or immunostained with antibodies against F4/80 and perilipin (Plin1). Bars=50 μm. Adipocyte size in H&E sections was quantified (n = 3).Values are expressed as mean ± SD. Unpaired two-tailed t-test between two groups (c) and two-way repeated-measures ANOVA followed by Bonferroni’s post hoc analysis (b, d) were conducted for statistical analyses. Source data are provided as a Source Data file.
