Extended Data Fig. 6: Effects of PAK4 overexpression on TG lipolysis in high fat diet-fed mice under fasting condition.
From: p21-activated kinase 4 counteracts PKA-dependent lipolysis by phosphorylating FABP4 and HSL
a. Schematic illustration of the preparation of PAK4- or PAK4S474A-loaded in vivo-jetPEI. Six-week-old male WT and Pak4 KO mice were injected with 20 μg of PAK4 or PAK4S474A and then fed an HFD for 9 weeks. b. Protein levels of Flag were examined in various organs at 9 weeks of HFD feeding in WT mice. c. PAK4 protein levels at indicated time points were compared in WT mice. d, h. Body weight change was monitored over time (n = 7 for d, n = 6 for h). *p < 0.05 versus mock. e-g, i-l. After 24-hour fasting, wet weights of EAT, plasma levels of FFA and glycerol and activation of lipolytic pathway in EAT were compared (n = 10 for e-g, n = 6 for i-k). Data are representative of at least three independent experiments. Values are mean ± SD. One-way ANOVA followed by Bonferroni’s post hoc analysis (e-g, i-k) and one-way repeated-measures ANOVA followed by Bonferroni’s post hoc analysis (d, h) were conducted for statistical analyses. Source data are provided as a Source Data file.
