Extended Data Fig. 1: Characteristics of diet-induced glucose intolerance and extracellular vesicle purity.
(A) Body weight over 20 weeks consumption of control (n = 30), NAFL (n = 15), and NASH diet (n = 15) across 1 cohort. *, significant from Control; #, significant from NASH. P < 0.001. (B) Liver and epididymal (EWAT) tissue mass in response to Control (n = 29), NAFL (n = 14), or NASH (n = 15 liver, n = 13 EWAT) across 1 cohort. *, significant from Control; #, significant from NASH. P < 0.001. (C-D) Blood glucose levels during an oral glucose tolerance test (2 g/kg) (C) and the corresponding glucose area under the curve (AUC; D) of mice fed different dietary interventions (n = 15 control, n = 9 NAFL, n = 13 NASH) across 1 cohort. *, significant from Control; #, significant from NASH. P < 0.001. (E) Serum insulin during the oral glucose tolerance test (n = 5 control, n = 9 NAFL, n = 9 NASH) across 1 experiment. *, significant from Control; #, significant from NASH. P = 0.01. (F) Steatosis score in Control (n = 8), NAFL (n = 10), and NASH (n = 10) livers derived from histopathology across 1 cohort. P = 0.1. (G) Lobular inflammation score in Control (n = 8), NAFL (n = 10), and NASH (n = 10) livers derived from histopathology across 1 cohort. P = 0.017. (H) Hepatocyte ballooning score in Control, NAFL, and NASH livers derived from histopathology (n = 8 control, n = 10 NAFL, n = 10 NASH) across 1 cohort. *, significant from Control; #, significant from NAFL. P < 0.001. (I) ATP / ADP ratio in liver slices and whole liver from Control, NAFL, and NASH mice (n = 6/group for liver slices, n = 9 for whole liver pooled from Control, NAFL, and NASH mice) across 1 experiment. P = 0.07. (J) Endotoxin levels in the circulation and in extracellular vesicle (EV) preparations (each bar represents an individual biological sample) across 1 experiment. Data are expressed as mean ± SEM. Each datapoint represents an independent biological sample. Data was analysed using a repeated measures two-way ANOVA (A, C, E) or one-way ANOVA (B (EWAT), D, F, H, I) followed by a Student Newman Keuls post-hoc, or Kruskal Wallis one-way ANOVA on ranks (B (liver), G) followed by a Dunn’s test.
