Extended Data Fig. 3: SAMS induction kinetics and specific metabolic response to distinct senescence inducers.
Fold changes kinetics of the indicated metabolites belonging to the SAMS in WI38 fibroblasts undergoing DDIS (A) or RAS-OIS (B). n = 3 biologically independent experiments for each time point and condition. Indicated p values were calculated using an unpaired two-sided Student’s t-test. Data are presented as mean values +/- SD. C: Sparse Least Squares Regression – Discriminant Analysis (sPLS-DA) depicting the two orthogonal components that maximize the separation of samples treated with different CS inducers. Metabolite levels were normalized by the Combat tool considering fibroblasts undergoing RAS- and RAF-OIS, DDIS, and RS. Dashed lines depict the metabolome trajectory for each treatment. The background colour depicts the predicted CS inducer given a sample metabolic state. D: Correlation circle depicting the projection of the sPLS-DA selected metabolites in each component. E: Normalized levels for the metabolites which activity best discriminate experimental treatment.
