Extended Data Fig. 10: GK upregulation in in-vivo models of senescence.
A: Levels of the indicated mRNAs normalized to those of Pinin as measured by RT–qPCR in mouse adipose tissue not expressing (PIK3CAWT) or expressing (PIK3CAAdipo-CreER) the constitutively active PI3KCA mutant. The reported values are relative to those of the PIK3CAWT genotype. Bars represent the means of n = 7 biological replicates (9 males and 5 females) +/- s.d. Indicated p values were calculated using an unpaired two-sided Student’s t-test. B: Left panel: Representative western blot showing the levels of the indicated proteins in mouse adipose tissue not expressing (PIK3CAWT) or expressing (PIK3CAAdipo-CreER) the constitutively active PI3KCA mutant. Right panel: Levels of the GK protein normalized to those of α-tubulin as measured on western blots on total protein extracts of mouse adipose tissue not expressing (PIK3CAWT) or expressing (PIK3CAAdipo-CreER) the constitutively active PI3KCA mutant. Values are normalized to those of the PIK3CAWT sample. Bars represent the means of 3 biological replicates +/- s.d (4 males and 2 females). Indicated p values were calculated using an unpaired two-sided Student’s t-test. C: Immunostaining with an anti-p21 and an anti-GK antibody on sections of the pancreas of WT mice (male) and of mice overexpressing in the pancreas the constitutively active G12D KRas-mutant (male). Asterisks indicate Langerhans islets. Scale bars represent 20 µm. The experiment was repeated independently twice with similar results.
