Fig. 8: Phosphoethanolamine and G3P accumulation are interconnected and regulate RB phosphorylation during senescence.
a, FC of G3P levels in WI38 fibroblasts infected with an adenovirus overexpressing a control shRNA (shCtrl) or an shRNA targeting the PCYT2 mRNA (shPCYT2) for 7 days, relative to the value of non-infected cells (Prolif.). n = 3 biologically independent experiments. Data are presented as mean ± s.d. Indicated P values were calculated using an unpaired two-sided Student’s t-test. b, FC of G3P levels and pEtN:CDP-Etn ratio in WI38 fibroblasts infected with GFP-OE or GK-OE adenovirus for 7 days, relative to the value of non-infected cells (Prolif.). n = 3 biologically independent experiments. Data are presented as mean ± s.d. Indicated P values were calculated using an unpaired two-sided Student’s t-test. c, FC of pEtN:CDP-Etn ratio in WI38 fibroblasts infected with GFP-OE or G3PP-OE adenovirus for 7 days, relative to the value of non-infected cells (Prolif.). n = 3 biologically independent experiments. Data are presented as mean ± s.d. Indicated P values were calculated using an unpaired two-sided Student’s t-test. d, Representative western blots showing indicated protein levels in WI38 fibroblasts not infected (Prolif.) or infected with GFP-OE or GK-OE adenoviruses for 4 or 7 days. Loading control (actin) was migrated into the same gel than p16, CCNA2 and GK. The experiment was repeated independently three times with similar results. e, Representative western blots showing indicated protein levels in WI38 fibroblasts not infected (Prolif.) or infected with an adenovirus overexpressing a control shRNA (shCtrl) or an shRNA targeting the PCYT2 mRNA (shPCYT2) for 7 days. The arrowhead indicates the position of the band corresponding to the PCYT2 protein. Loading control (actin) was migrated into the same gel as CCNA2 and RB. The experiment was repeated independently three times with similar results. f, Representation of G3P and pEtN metabolic interconnections leading to TAG accumulation and senescence.
