Extended Data Fig. 1: Transcriptome Evolution of Senescence Inducers.

A: Heat maps showing modules of temporally coexpressed genes for the indicated senescence inducers and quiescence (Q) at the indicated time points using an unsupervised weighted clustering network analysis (WGCNA) approach. Roman numerals refer to different gene clusters. Data are expressed as row Z scores collected from two biologically independent experiments per condition. B: Functional over-representation map depicting Molecular Signaling Database(MSigDB) hallmark (H.) gene sets associated with each transcriptomic cluster for the indicated senescence inducers. Circles are colour-coded according to the FDR-corrected p value based on the hypergeometric text comparing the overlap between the set of genes in each cluster and the respective list of genes in each MSigDB pathway. Size is proportional to the percentage of genes in the MsigDB gene set belonging to the cluster. N > 100 genes per transcriptomic module for each senescence inducer. Exact values for raw p values, adjusted p values and overlap (absolute and relative) between each pair of sets are reported in Supplementary Table S2. C: Expression heat map of core senescence genes for the indicated senescence inducers and quiescence as control.