Extended Data Fig. 3: Bcl6 controls an oxidative to glycolytic shift in myofibres.

(a) Representative fluorescent antibody-stained images of four myosin heavy chain isoforms in quadriceps from Cre^- tmx and Bcl6^i-MKO mice (left). MYH7 (type 1 fiber) is blue, MYH2 (type 2a fiber) is green, MYH1 (type 2x fiber) is black, and MYH4 (type 2b fiber) is red. Quantification of fiber types (right) expressed as percentage of total (n = 3/group). Cre^- tmx vs Bcl6^i-MKO type 2a fiber P = 0.0374 by unpaired, two-tailed t-test with Welch correction. (b) qPCR DNA ratios of mitochondrial genes Cox1 and Cytb over nuclear genes Gcg and Hbb in quadriceps from Bcl6^fl/fl and Bcl6^MKO males (n = 7/group). (c) Western blot and densitometry of VDAC and total (Licor) protein in quadriceps from n = 6 Bcl6^fl/fl and n = 7 Bcl6^MKO males. (d) Protein levels of mitochondrial complex subunits and total (Memcode) protein in quadriceps from Bcl6^fl/fl and Bcl6^MKO mice. Western blot (left) and protein densitometry (right) are shown. (n = 4/group) Bcl6^fl/fl vs Bcl6^MKO complex II P = 0.0139 by unpaired, two-tailed t-test with Welch correction. (e, f) Cross-sectional areas of specific fiber types determined from fluorescent antibody-stained sections in quadriceps from (e) Bcl6^fl/fl and Bcl6^MKO mice (n = 3/group) and (f) Cre^- tmx and Bcl6^i-MKO mice (n = 3/group). Type 2b fiber in Bcl6^fl/fl vs Bcl6^MKO P = 0.003 and in Cre⁻ tmx vs Bcl6^i-MKO P = 0.0385 by unpaired, two-tailed t-test with Welch correction. All data are represented as mean + SEM. * p < 0.05, ** p < 0.01.

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