Extended Data Fig. 1: Bcl6 controls the development of skeletal muscle mass.
From: Transcriptional programming of translation by BCL6 controls skeletal muscle proteostasis
(a) qPCR expression of Bcl6 and myogenic markers in primary myoblasts and myotubes relative to the housekeeping gene Rn45s (n = 4 per group). Myoblast vs myotube P-value for Bcl6 = 0.0124, Mb = 0.0153, Mef2c = 0.0146 by unpaired, two-tailed t-test with Welch correction. (b) Western blot and densitometry of BCL6 and total (Memcode) protein in primary myoblasts and myotubes (n = 4/group). Myoblast vs myotube P = 0.0036 by unpaired, two-tailed t-test with Welch correction. (c) Western blot and densitometry of BCL6 and Actin protein in heart from Bcl6fl/fl and Bcl6MKO mice (n = 3/group). (d) Total body weight, fat, and lean mass in n = 7 Bcl6fl/fl and n = 10 Bcl6MKO female mice at 8 weeks of age. Bcl6fl/fl vs Bcl6MKO lean mass P = 0.0184 by unpaired, two-tailed t-test with Welch correction. (e) Tissue weights of quadriceps (quad), gastrocnemius (gastroc), tibialis anterior (TA), extensor digitorum longus (EDL) and soleus from female Bcl6fl/fl and Bcl6MKO mice at 16 weeks of age (n = 7/group). Unpaired, two-tailed t-test with Welch correction was performed and all p-values can be found in the source data file. All data are represented as mean + SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001.
