Extended Data Fig. 6: Lactate is not sufficient to increase excitability of spinal L1 neurons in the absence of PTG (related to Fig. 6).

(a) Rheobase comparison in naïve gPTG^−/− neurons recorded with the addition of lactate (15 mM) into recording aCSF. N = 23 cells/N = 4 animals (gPTG^−/−) and N = 26/3 (gPTG^−/− + Lactate). (b) Rheobase comparison in CFA-treated gPTG^−/− group recorded with the addition of lactate (15 mM) or pyruvate (15 mM) into recording aCSF. N = 19/4 (gPTG^−/−+CFA), N = 28/3 (gPTG^−/−+CFA+Lactate) and N = 28/3 (gPTG^−/−+CFA+Pyruvate). (c) Comparison of the firing frequencies in response to 500 ms current injections (from 0pA to 120pA above rheobase) in naïve gPTG^−/− neurons in the presence of L-lactate. N = 23/4 (gPTG^−/−) and N = 26/3 (gPTG^−/− + Lactate). (d) Comparison of the firing frequencies in response to 500 ms current injections (from 0pA to 120pA above rheobase) in CFA gPTG^−/− neurons in the presence of L-lactate or pyruvate. N = 19/4 (gPTG^−/−+CFA), N = 28/3 (gPTG^−/−+CFA+Lactate) and N = 28/3 (gPTG^−/−+CFA+Pyruvate). (e) Comparison of firing frequencies at 50pA above rheobase current (based on b). N = 23/4 (gPTG^−/−) and N = 26/3 (gPTG^−/− + Lactate). (f) Comparison of firing frequencies at 50pA above rheobase current (based on e). N = 19/4 (gPTG^−/−+CFA), N = 28/3 (gPTG^−/−+CFA+Lactate) and N = 28/3 (gPTG^−/−+CFA+Pyruvate). (g) Example traces of firing patterns of gPTG^−/−/gPTG^−/−+CFA neurons recorded in the presence of lactate and pyruvate. Data shown as mean ± s.e.m. Boxes in panels a, b, e and f extend from the 25^th to 75^th percentiles, whiskers indicate smallest and largest values, center lines represent median.