Fig. 2: Inflammatory pain induces glycogen accumulation in the dorsal spinal cord. | Nature Metabolism

Fig. 2: Inflammatory pain induces glycogen accumulation in the dorsal spinal cord.

From: Neuron–astrocyte metabolic coupling facilitates spinal plasticity and maintenance of inflammatory pain

Fig. 2

a, Glycogen content of ipsilateral and contralateral dorsal spinal cord tissue isolated at different time points after FA stimulation and expressed as percentage of glycogen of ipsilateral tissue isolated from naive mice (n = 6 for naive; n = 3 for 2 h; n = 8 for 6 h; n = 3 for 24 h and 72 h); two-way ANOVA with Bonferroni post hoc test. b,c, Representative images of in situ hybridizations (RNAscope) with a probe for Ptg (red) of spinal cord tissue 2 h after capsaicin stimulation (b) or CFA stimulation (c) and DAPI (blue); scale bars, 50 µm. d,e, Glycogen content (percentage of naive) of dorsal spinal cord tissue at different time points after capsaicin stimulation (d), with n = 5 mice for naive and n = 7 for 6 h and n = 2 for 1 d, or CFA stimulation (e), with n = 6 for naive, n = 5 for 30 min, n = 6 for 1d, n = 3 for 7 d and n = 4 for 10 d; one-way ANOVA with Tukey’s post hoc test. Data represent mean ± s.e.m. See also Extended Data Fig. 2.

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