Fig. 3: Noxious stimulation-induced spinal glycogen dynamics is blunted in PTG−/− mice. | Nature Metabolism

Fig. 3: Noxious stimulation-induced spinal glycogen dynamics is blunted in PTG−/− mice.

From: Neuron–astrocyte metabolic coupling facilitates spinal plasticity and maintenance of inflammatory pain

Fig. 3

a, Representative confocal images of in situ hybridizations (RNAscope) with a probe against Ptg (green) in the ipsilateral dorsal spinal cord tissue from WT, cPTG−/− and gPTG−/− mice 2 h after CFA-induced pain, and its colocalization with the neuronal marker (NeuroTrace) and an astrocytic marker (GFAP) in blue and red, respectively. Scale bars, 20 µm. b, Quantification of Ptg mRNA signals visualized by RNAscope in WT and PTG KO models as shown in a (quantified are the number of Ptg ‘dots’ in 3 images from n = 3 animals per genotype). c,d, Glycogen content (expressed as percentage of glycogen content measured in dorsal spinal cord tissue of naive WT control mice) at different time points after CFA stimulation of gPTG−/− mice (c, n = 5 samples of 5 mice) and cPTG−/− mice (d, n = 5 samples of 5 mice) compared with their respective control mice. Two-way ANOVA with Bonferroni post hoc test. Data represent mean ± s.e.m. See also Extended Data Fig. 3.

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