Fig. 4: Inflammatory nociceptive sensitization and pain maintenance is reduced in PTG^−/− mice.

a,b, Baseline mechanical sensitivity for WT and gPTG^−/− mice (a, n = 6) or PTG^+/fl and cPTG^−/− mice (b, n = 12). c,d, Mechanical threshold required to elicit a response in at least 60% of trials in PTG^+/fl and cPTG^−/− mice either not treated (baseline) or at 15 min after intraplantar serotonin (c, n = 6) or PGE2 (d, n = 6) injection into the hindpaw. e,f, Time course of FA-induced nocifensive responses scored in 5-min bins (e) or separated into Phase I (0–10 min) and Phase II (10–50 min) (f) for WT and gPTG^−/− mice (n = 5). g,i,k, The 60% mechanical threshold measured before and at different time points after CFA stimulation comparing WT (n = 5) and gPTG^−/− mice (g, for 7 d **P = 0.0092; for 14 d ****P < 0.0001; for 21 d **P = 0.0025; n = 5), PTG^+/fl and cPTG^−/− mice (i, for 7 d *P = 0.013; for 14 d ****P < 0.0001; for 21 d *P = 0.013; n = 12), and AAV-Control- (pAAV.GFAP.eGFP.WPRE.hGH, n = 9) and AAV-GFAP-Cre- (pssAAV-2-hGFAP-mCherry_iCre-WPRE-hGH, n = 7) injected PTG^fl/fl mice (k, for 6 d *P = 0.0252; for 7 d *P = 0.0216; for 14 d **P = 0.0048). h,j,l, Number of days required to recover from a sensitized state back to baseline mechanical threshold (cutoff: 1 s.d. of the average baseline value) after intraplantar CFA injection of WT and gPTG^−/− mice (h), PTG^+/fl and cPTG^−/− mice (j), and AAV-Control- and AAV-GFAP-Cre-injected PTG^fl/fl mice (l). Data correspond to traces shown in g, i and k. a–g, i and k, two-way ANOVA with Bonferroni post hoc test; h, j and l, unpaired two-tailed t-test. Data represent mean ± s.e.m. In all behaviour experiments littermates were used as controls. See also Extended Data Fig. 4.