Extended Data Fig. 5: Lactate induces cachectic phenotype in mice, while UCP1 ablation ameliorates LLC-induced cachexia.
From: Activation of GPR81 by lactate drives tumour-induced cachexia
a, Representative anatomical images and hematoxylin and eosin (H&E) staining of inguinal white adipose tissue (iWAT) from mice implanted with osmotic minipumps loaded with PBS or sodium L-lactate for 7 days. Scale bars, 1 cm in anatomical images (red bar in upper panel) and 100 μm in H&E staining (black bars in lower panels). b,c, Body weight (b) and tissue weights (c) of mice 7 days after implanted with osmotic minipumps loaded with PBS or sodium L-lactate (n = 5 for each group). d, Representative UCP1 immunohistochemical staining of the iWAT from mice 7 days after implanted with osmotic minipumps loaded with PBS or sodium L-lactate (n = 5 for each group). Scale bars, 100 μm. e, Representative western blots showing UCP1 protein level in the brown adipose tissue (BAT) from WT and UCP1 knockout (UCP1−/−) mice (n = 4). f, Representative anatomical images of iWAT from WT control, WT tumour-bearing (TB) and UCP1-/- TB mice. Scale bar, 1 cm. g, Weight of tumours derived from WT TB and UCP1-/- TB mice (n = 4). All data are presented as the mean ± s.e.m. In panel d, P value was determined by two-tailed unpaired Student’s t-test (b, c, g).
