Extended Data Fig. 7: Adipose GPR81 plays an important role in tumour-induced WAT browning and inflammation.
From: Activation of GPR81 by lactate drives tumour-induced cachexia
a, GSEA identified WAT browning-related gene sets were significantly down-regulated in the inguinal white adipose tissue (iWAT) of GPR81 knockout tumour-bearing (GPR81-/- TB) relative to the wild type tumour-bearing (WT TB) mice. (n = 3 for each group). b, TNF signaling-related gene set was down-regulated in the iWAT of GPR81-/- TB as compared with WT TB (n = 3 for each group). c,d, The levels of TNF-α (c), IFN-γ, IL-6, and IL-1β (d) in the iWAT assessed by ELISA (n = 4 for each group). e, Relative GPR81 mRNA levels in the iWAT from WT and GPR81-/- mice (n = 14 for each group) and their quadriceps (n = 7 for each group). f, Lactate levels of the iWAT interstitial fluid at baseline, 12 and 24 days after xenoplanted with LLC cells subcutaneously (n = 5 for each group). g, Linear regression analysis showing the correlation between the iWAT weight with iWAT interstitial fluid lactate level 24 days after xenoplanted with LLC cells (n = 17). h, Statistic results showing GPR81 mRNA levels in the iWAT from GPR81fl/fl and GPR81fl/flAdipoqcre mice (n = 8 for each group). All data are presented as the mean ± s.e.m. P values were determined by one-way ANOVA with Tukey’s multiple comparisons test (c, d, f) and two-tailed unpaired Student’s t-test (e). P value of the correlation was determined by two-tailed Pearson correlation analysis (g).
