Extended Data Fig. 1: Differential serum metabolites in tumour-free versus tumour-bearing mice.
From: Activation of GPR81 by lactate drives tumour-induced cachexia
a, Tissue weights of inguinal white adipose tissue (iWAT), gonad white adipose tissue (gWAT), and quadriceps of tumour-free (Control) and xenograft tumour-bearing mice (LLC-TB) 24 days after injected with LLC cells (n = 5 for each group). b, Partial Least Squares Discriminant Analysis (PLS-DA) of untargeted metabolomics profiling of serum samples from the LLC-TB and Control mice (n = 4 for each group). c-e, The retention time (c), m/z value (d), and calibration curve (e) of lactate standard in mass spectrum. f, Blood lactate levels of Control and LLC-TB mice on day 0, 12 and 24 days after injecting PBS or LLC cells (n = 5 for each group). g, Linear regression analysis showing the correlation between blood lactate level and percentage of body weight change in the mice xenograft with LLC cells (n = 41). h, Relative amount of 13C-labeled lactate in the blood and major organs of mice with or without tumour (n = 5). All data are presented as the mean ± s.e.m. P values were determined by two-tailed unpaired Student’s t-test (a, h), two-way ANOVA (f). P value of the correlation was determined by two-tailed Pearson correlation analysis (g).
