Extended Data Fig. 2: Cachectic phenotypes in orthotopic and genetically engineered mouse models of lung cancer.
From: Activation of GPR81 by lactate drives tumour-induced cachexia
a-c, Orthotopic lung tumours induced an increase in blood lactate level (a), while decreases in body weight (b) and tissue mass of inguinal white adipose tissue (iWAT), gonad white adipose tissue (gWAT) and quadriceps (c) in mice (n = 5 for each group). d-f, Changes in tissue weights of iWAT, gWAT, brown adipose tissue (BAT), quadriceps (d); body fat percentage (e); and grip strength (f) of KrasLSL-G12D/+;p53R172H/+ mice after intranasal administration of control adenovirus (KrasLSL-G12D/+;p53R172H/+;Null, n = 5) or adenovirus expressing Cre recombinase (KrasLSL-G12D/+;p53R172H/+;Cre, n = 4). g, h, Energy expenditure (EE) over 24 h (g) and regression-based analysis of total daily EE against body weight (h) of KrasLSL-G12D/+;p53R172H/+ mice 8 weeks after adenovirus administration (n = 4). All data are presented as the mean ± s.e.m. P values were determined by two-tailed unpaired Student’s t-test (a, c, d, f), two-way ANOVA (b, e), or ANCOVA (h).
