Extended Data Fig. 7: Expression and function of AstC-R1+ enteric neuron on the proventriculus.
From: Postprandial sodium sensing by enteric neurons in Drosophila
a A confocal image of anterior enteric neurons (green) labeled by AstC-R1-GAL4 crossed to UAS-CD8::GFP, and counterstained with phalloidin (blue). Scale bars, 20 μm. n = 5. b Behavioral responses of AstC-R1-GAL4 crossed to tub-GAL80ts, UAS-kir2.1 for inactivation of the anterior enteric neurons that were sated, 72-hour deprived, or refed with 10 mM NaCl for 6 and 24 hours prior to the two-choice assay. N = 5. c, d. Representative images of AstC-R1+ neurons in the brains of flies carrying AstC-R1-GAL4, UAS-tdtomato and UAS-CaLexA that were deprived (c) and re-sated with 50 mM NaCl (d). Native tdtomato (magenta) and GFP (green) fluorescence signals were visualized. Scale bars, 50 μm. n = 4 Data showed as mean values ± s.e.m. Individual p values are shown in the source data file. ***p < 0.001, **p < 0.01: one-way ANOVA with Tukey’s post-hoc test.
