Extended Data Fig. 8: INSO split-GAL4 are response to sodium after deprivation.
From: Postprandial sodium sensing by enteric neurons in Drosophila
a-e. Representative images of INSO neurons of flies carrying Split-GAL4, UAS-tdtomato and UAS-CaLexA, that were deprived (a), re-sated by the intake of NaCl (b), the intake of NaHCO3, MSG or Na2H2PO4 (c), and the intake of CaCl2, MgCl2 or NMDG-Cl (d), and the quantification (e) of CaLexA-derived native GFP fluorescence signals from INSO neurons of deprived, re-sated with denoted sodium-containing and chloride-containing salts. Native tdtomato (magenta) and GFP (green) fluorescence, counterstained with DAPI (blue) were illuminated. Dash lines outline the cell bodies of INSO neurons. Scale bars, 5 μm. n = 5–6. Data showed as mean values ± s.e.m. Individual p values are shown in the source data file. **p < 0.01, *p < 0.05, ns: one-way ANOVA with Dunnett’s multiple comparisons test.
