Extended Data Fig. 4: Cluster analysis of stromal vascular cells.
Single-cell RNA sequencing of epidydimal stromal vascular cells from WT 4 months HFD/obese mice treated for the last month with 3 mg kg-1 d-1 rosiglitazone (Rosi) compared to HFD control mice: a, Heat map showing scaled counts per million expression values of marker genes for broad clusters: macrophages, DCs, T cells, B cells, mast cells, endothelial cells, and adipocyte progenitors. b, Heat map showing genes involved in PPAR signalling pathways of broad macrophage cluster. c, d, Up- (c) or downregulated (d) GO Biological Process 2021 pathways after Rosi treatment compared to HFD for the macrophage cluster (FDR< = 0.1). Point sizes reflect the combined EnrichR score, and the colour represents the P-value for each annotated pathway analysed by two-tailed Wilcoxon rank-sum test. e, Heat map showing scaled counts per million expression values of macrophage subpopulation marker genes in each subcluster: Monocyte-derived (Mac5), ‘M1-like’ (Mac3), lipid-associated (LAMs/Mac2), and ‘M2-like’ ATMs (Mac1 and Mac4). Data are representative of three biologically independent mice from one experiment/cohort (n = 3 per group).
