Fig. 1: Mapping the temporally resolved core proteome of human adipogenesis.
a, LC–MS-based proteomics workflow for mapping the core proteome of human adipogenesis. Proteomic signatures of four human adipogenesis models at multiple time points (n = 3) were compared with proteomes from human WAT, pACs and SVFs from seven patients. Image was partially created with BioRender.com. b, Dynamic range of cell models and primary cell proteomes. c, PCA of primary samples and the differentiation stages of the cell lines (depicted as light to dark). Protein filtered for at least two valid values in all cell models and primary samples. d, PCA loadings with major driver proteins involved in lipid metabolism shown in red. e, Overlap of proteins significantly changed during differentiation in each of the models (individual ANOVA tests for each model, FDR < 10−2). f, Supervised hierarchical clustering of the z-scored temporal profiles of the 3,934 significantly changed proteins in at least three of the four models, as outlined in e. g, Supervised hierarchical clustering of z-scored temporal profiles of all cell models of a subset of f with conserved temporal profiles (Pearson correlations of inter-cell model comparison for each protein’s temporal profiles >0). The protein levels of all four models are shown next to each other at the indicated time points. h, Profiles of individual clusters and a selection of enriched annotations (one-sided Fisher’s exact test, enrichment score >2, Benjamini–Hochberg FDR < 0.1). Enrichment values and P values are depicted as bubble size and colour code, respectively.
