Extended Data Fig. 9: The LD proteome of human white adipocytes.
(a) Supervised hierarchical clustering of temporal profiles of significantly altered LD proteins during adipogenesis. Hierarchical clustering of PCPs of hAPCs (n = 1). Enriched GO-terms in the clusters are indicated (Fisher’s test, FDR < 0.1). (b) Profiles of the indicated proteins involved in de novo lipogenesis in hAPCs overlaid with the marker profiles of cytosolic proteins and proteins from protein complexes. (c) and (d) Filtering of LD-assigned and LD-enriched proteins versus the total proteome. Protein levels of proteins determined as LD proteins as first or second assignments from SVMs based on PCP analysis in hAPCs in the LD fraction versus the total proteome from SGBS and hAPCs, respectively. (e) Functional clusters of LD proteins mapped in both adipocyte models were analysed using the STRING database, and those with at least two partners (physical and/or functional) are shown. High-confidence interactions with thicker connected lines. GO-enriched annotation terms (FDR < 5*10^(−2)) are coloured to highlight specific clusters. (Size = number of connections; line width = STRING combined score).
