Fig. 1: Transcriptional regulation of cAMP biosynthesis during cold adaptation of adipose tissue.
From: Cold-induced expression of a truncated adenylyl cyclase 3 acts as rheostat to brown fat function
a–c, Volcano plots of significantly upregulated (magenta) and downregulated (blue) genes in BAT (a; n = 2,290; 1,086/1,204 upregulated/downregulated differentially expressed genes (DEGs)), iWAT (b; n = 1,252; 804/448 upregulated/downregulated DEGs) and eWAT (c; n = 655 upregulated/downregulated DEGs) in 20-week-old C57BL/6N (a and c) or C57BL/6J (b) male mice at RT (22 °C) or after 24 h (a and c) and 72 h (b) of 5 °C CE, determined by mRNA-seq. Statistically significant DEGs were defined as false discovery rate (FDR) ≤ 0.05 and log transcripts per million (TPM) > 0. FC, fold change. N = 3–5 animals per temperature condition were analysed. d–f, KEGG pathway enrichments (Benjamini & Hochberg-corrected P value ≤ 0.05) for DEGs shown in a–c. g–i, mRNA expression of AC (Adcy) isoforms in BAT (g), iWAT (h) and eWAT (i), determined by mRNA-seq and depicted in TPM. N = 3–5 animals per temperature condition were analysed. Bar graphs represent the mean + s.e.m. with all data points plotted. Unpaired, two-tailed and non-parametric Mann–Whitney tests were performed to assess statistical significance. P values are indicated. j,k, Analysis of public snRNA-seq data (E-MTAB-8562) from Adipoq-tdTomato-positive adipocyte nuclei isolated from male mice housed at TN, RT and after CE at 8 °C for 4 days. The colours in j depict expression of Adcy3 (green), Ucp1 (red) or both (yellow). k, Ridgeline plots depicting frequency (in nuclei) of expression of BAT identity genes at indicated temperatures.
