Extended Data Fig. 4: Biochemical principles recapitulated by DMS.
From: Systematic analysis of NDUFAF6 in complex I assembly and mitochondrial disease
a-d. DMS fitness scores of substitutions in alanine residues (a), aromatic residues (b), hydrophobic residues (c), and charged residues (d). BLOSUM62 similarity scores for each substitution is shown along the right. Predicted secondary structure is shown below, conservation (calculated using ConSurf), and percent solvent accessible surface area (% SASA) are shown below. e. Surface representation of AF6 AlphaFold model colored by conservation, mutational sensitivity, and electrostatics. f. Binned scatterplot showing the distribution of conservation (calculated by ConSurf) and mutational sensitivity of residues in AF6. The dot size represents the number of residues in each bin.
