Extended Data Fig. 7: The ability of Bv-ΔspeA to induce PCOS-like phenotype is significantly reduced compared to B. vulgatus.
a, The percentage Bacteroides vulgatus strains with or without speA. b, The relative abundance of speA gene in the metagenomic dataset from PMID: 31332392. n = 25 in Control (BMI < 25) group; n = 25 in PCOS (BMI < 25) group; n = 18 in Control (BMI ≥ 25) group; n = 25 in PCOS (BMI ≥ 25) group. For box plots, the midline represents the median; box represents the interquartile range (IQR) between the first and third quartiles, and whiskers represent the minimum to maximum values. Bacteroides vulgatus: Control (BMI < 25) vs PCOS (BMI < 25) P = 2.56 × 10−7, Control (BMI ≥ 25) vs PCOS (BMI ≥ 25) P = 0.000015; Bifidobacterium longum: Control (BMI ≥ 25) vs PCOS (BMI ≥ 25) P = 0.000025. c, DNA gel electrophoresis experiment to verify the knockout of Bv-ΔspeA in B. vulgatus. d, Growth curve of wild type B. vulgatus (Bv) and Bv-ΔspeA in BHI medium. e-m, SPF mice were gavaged with Bv or Bv-ΔspeA for three weeks. e, Colonization of Bv and Bv-ΔspeA in mice. f, Quantitative analysis of corpora lutea in the ovaries. g, Quantitative analysis of cystic follicles in the ovaries. h, Levels of testosterone in serum. i, Levels of LH in serum. j, Area under the curve (AUC) of GTT. k, The levels of fasting glucose in serum. l, The level of insulin in serum. m, HOMA-IR. All data are presented as the mean ± s.e.m. (d) n = 3/group. (e, h-m) n = 6/group. (f, g) n = 5/group. In b, the P values were determined by Kruskal–Wallis test followed by Dunn’s post hoc test. In d, i-m, the P values were determined by two-tailed Student’s t-test. In e-h, the P values were determined by two-tailed Mann–Whitney U-test.
