Extended Data Fig. 6: Physical Activity during the Active Phase Stimulates Bone Growth through the Process of Oxidative Phosphorylation.
From: Time of exercise differentially impacts bone growth in mice
a, The schematic shows mice injected with oxidative phosphorylation inhibitor (oligomycinA, Selleckchem, 15 mg/kg/d, intraperitoneal injection) or equal solvent 60 minutes before exercise. b, Representative images of micro-CT reconstruction of femora from Sed and Exe mice with or without oxidative phosphorylation inhibitor injection. c, Micro-CT analysis of the total femora length, BV/TV, Tb.Th, and Tb.N of Sed and Exe mice injected oxidative phosphorylation inhibitor or equal solvent (n = 5 biologically independent experiments). d, e, Representative images of S-O staining of the distal femora in Sed and Exe mice injected with oxidative phosphorylation inhibitor or equal solvent (n = 3 biologically independent experiments). Scale bar, 50 μm. f, g, EdU staining of proliferating cells in PZ from the growth plate cartilages in Sed and Exe mice injected oxidative phosphorylation inhibitor or equal solvent. Arrowheads indicate EdU+ cells (n = 3 biologically independent experiments). Scale bar, 50 μm. c, e, g, Data were presented as mean ± SD and analyzed using two-way ANOVA with the Tukey multiple comparisons test.
