Extended Data Fig. 7: Physical Activity during the Active Phase Stimulates Bone Growth through the Process of Oxidative Phosphorylation.
From: Time of exercise differentially impacts bone growth in mice
a, Adenosine triphosphate (ATP) production of femur in Sed and Exe mice intraperitoneally injected oxidative phosphorylation inhibitor or equal solvent (n = 3 biologically independent experiments). b, The content of NAD+ and NADH, and the ratio of NAD + / NADH of femur in Sed and Exe mice injected oxidative phosphorylation inhibitor or equal solvent (n = 3 biologically independent experiments). c, d, Western blot analysis of the levels of ATP6V0D1, Cyclin D2, SOX9 and COL2α1 in the femoral metaphysis tissue from Sed and Exe mice injected oxidative phosphorylation inhibitor (oligomycin A) or equal solvent during the early rest versus active phase (n = 3 biologically independent experiments). e, f, Immunofluorescence of COL2α1 and ACAN in the growth plate cartilages after exercise during the early rest versus active phase with or without oxidative phosphorylation inhibitor injection. Scale bars, 50 μm. One technical replicate of three biological replicates for each group. a, b, d, Data were presented as mean ± SD and analyzed using two-way ANOVA with Tukey multiple comparisons test.
