Extended Data Fig. 10: Stimulating Oxidative Phosphorylation Pharmacologically during the Early Active Phase Enhances Bone Growth.
From: Time of exercise differentially impacts bone growth in mice
a, Representative images of micro-CT reconstruction of femora from Sed and Exe mice with or without oxidative phosphorylation activator injection (AICAR, Selleckchem, 50 mg/kg/d, a total of 28 days, intramedullary injection). b, Micro-CT analysis of the total femora length, BV/TV, Tb.Th, and Tb.N of Sed and Exe mice intramedullary injected oxidative phosphorylation activator or equal solvent (n = 5 biologically independent experiments). c, d, Representative images of S-O staining of the distal femora in Sed and Exe mice intramedullary injected with oxidative phosphorylation activator or equal solvent (n = 3 biologically independent experiments). Scale bar, 50 μm. e, f, EdU staining of proliferating cells in PZ from the growth plate cartilages in Sed and Exe mice intramedullary injected oxidative phosphorylation activator or equal solvent. Arrowheads indicate EdU+ cells (n = 3 biologically independent experiments). Scale bar, 50 μm. g, h, Immunofluorescence of COL2α1 and ACAN in the growth plate cartilages after exercise at the early rest versus active phase with or without oxidative phosphorylation activator intramedullary injection. Scale bars, 50 μm. b, d, f, Data were presented as mean ± SD and analyzed using two-way ANOVA with the Tukey multiple comparisons test.
