Extended Data Fig. 3: Additional Analysis of Apoe-/- Mice Exposed to IHC Conditions.
From: Time of sample collection is critical for the replicability of microbiome analyses
A) Weighted UniFrac PCoA stacked view (same as Fig. 2b but different orientation). Good for assessing overall similarity not broken down by time point. Significance determined by PERMANOVA (p = 0.005). B) Weighted UniFrac PCoA of only axis 1 over time. C) Boxplot/scatterplot of within-group weighted UniFrac distance values for the control group (Air, n = 3–4 samples per time point). Unique non-zero values in the matrix were kept. Dotted line indicates the mean of all values presented. No significant differences (p > 0.05) found. D) Boxplot/scatterplot of within-group weighted UniFrac distance values for the experimental group (IHC, n = 3–4 samples per time point)). Unique non-zero values in the matrix were kept. Dotted line indicates the mean of all values presented. No significant differences (p > 0.05) found. E) Boxplot/scatterplot of within-group weighted UniFrac distance values for both control (Air) and experimental (IHC) groups [n = 3–4 samples per group per time point]. Mann-Whitney-Wilcoxon test with Bonferroni correction used to determine significant differences between groups. Boxplot centre line indicates median, edges of boxes are quartiles, error bars are min and max values. Notation: ns = not significant, p > 0.05; * = p < 0.05; ** = p < 0.01; *** = p < 0.001.
